7 research outputs found
Estrogen Induction of Telomerase Activity through Regulation of the Mitogen-Activated Protein Kinase (MAPK) Dependent Pathway in Human Endometrial Cancer Cells
Get PDFGiven that prolonged exposure to estrogen and increased telomerase activity are associated with endometrial carcinogenesis, our objective was to evaluate the interaction between the MAPK pathway and estrogen induction of telomerase activity in endometrial cancer cells. Estradiol (E2) induced telomerase activity and hTERT mRNA expression in the estrogen receptor (ER)-α positive, Ishikawa endometrial cancer cell line. UO126, a highly selective inhibitor of MEK1/MEK2, inhibited telomerase activity and hTERT mRNA expression induced by E2. Similar results were also found after transfection with ERK 1/2-specific siRNA. Treatment with E2 resulted in rapid phosphorylation of p44/42 MAPK and increased MAPK activity which was abolished by UO126. The hTERT promoter contains two estrogen response elements (EREs), and luciferase assays demonstrate that these EREs are activated by E2. Exposure to UO126 or ERK 1/2-specific siRNA in combination with E2 counteracted the stimulatory effect of E2 on luciferase activity from these EREs. These findings suggest that E2-induction of telomerase activity is mediated via the MAPK pathway in human endometrial cancer cells
<html>Increased efficacy of metformin corresponds to differential metabolic effects in the ovarian tumors from obese <i>versus</i> lean mice</html>
Get PDFObesity is a significant risk factor for ovarian cancer (OC) and associated with worse outcomes for this disease. We assessed the anti-tumorigenic effects of metformin in human OC cell lines and a genetically engineered mouse model of high grade serous OC under obese and lean conditions. Metformin potently inhibited growth in a dose-dependent manner in all four human OC cell lines through AMPK/mTOR pathways. Treatment with metformin resulted in G1 arrest, induction of apoptosis, reduction of invasion and decreased hTERT expression. In the K18-gT121+/-; p53fl/fl; Brca1fl/fl (KpB) mouse model, metformin inhibited tumor growth in both lean and obese mice. However, in the obese mice, metformin decreased tumor growth by 60%, whereas tumor growth was only decreased by 32% in the lean mice (p=0.003) compared to vehicle-treated mice. The ovarian tumors from obese mice had evidence of impaired mitochondrial complex 2 function and energy supplied by omega fatty acid oxidation rather than glycolysis as compared to lean mice, as assessed by metabolomic profiling. The improved efficacy of metformin in obesity corresponded with inhibition of mitochondrial complex 1 and fatty acid oxidation, and stimulation of glycolysis in only the OCs of obese versus lean mice. In conclusion, metformin had anti-tumorigenic effects in OC cell lines and the KpB OC pre-clinical mouse model, with increased efficacy in obese versus lean mice. Detected metabolic changes may underlie why ovarian tumors in obese mice have heightened susceptibility to metformin
The effect of E2 on telomerase activity and hTERT expression in the ER-positive Ishikawa cell line.
No full text<p>Cells were treated with different concentrations of E2 (0.1–10 µM) for 48 hr or treated with 1 uM E2 in a time-course fashion (A). Telomerase activity was determined by the TRAP assay. hTERT RNA expression was assessed by real-time RT-PCR (B). The data are presented as means ± SD of duplicated samples from at least two independent experiments. (ITAS = internal telomerase assay standard, C = control).</p
The effect of estrogen and UO126 on hTERT promoter activity.
No full text<p>Schematic diagram of hTERT promoter luciferase plasmids showing two ERE binding sites and core promoter (A). Ishikawa cells were transfected with hTERT promoter luciferase plasmids and luciferase activity was assayed after exposure to 1 uM E2 or 10 uM UO126 (U) for 36 hr. The data are presented as means ± SD of duplicated samples from at least two independent experiments. (C = control).</p
The effects of ERK1/2-specific siRNA in combination with estrogen on telomerase, hTERT expression and hTERT promoter activity in Ishikawa cells.
No full text<p>The cells were either transfected with ERK1/2 siRNA or negative control (Neg) for 8 hr and then treated with 1 uM estrogen for 36–48 hr. The effect of transfection with ERK 1/2-specfic siRNA on phosphorylation for p42/p44 induced by estrogen was assessed by Western blotting at 48 hr (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055730#pone-0055730-g006" target="_blank">Figure 6A</a>). Telomerase activity and hTERT expression were assayed by TRAP assay and real time RT-PCR at 48 hr (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055730#pone-0055730-g006" target="_blank">Figure 6B and 6C</a>). Lucifersae activity was assayed after cells were transfected with ERK1/2 siRNA for 24 hr, followed by transfection with the hTERT promoter luciferase plasmid (P3915) and then treatment with 1 uM estrogen for 36 hr (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055730#pone-0055730-g006" target="_blank">Figure 6D</a>). (ITAS = internal telomerase assay standard, C = control).</p
The effect of UO126 on telomerase activity and hTERT mRNA expression.
No full text<p>The Ishikawa cells were treated with UO126 at varying concentrations (0.1–10 µM) for 24 hr. Telomerase activity was assessed by TRAP assay (A and B) hTERT expression was assessed by real time RT-PCR (C). (C = control).</p
